Evolução e aspectos moleculares de pacientes com Covid-19 em hospital público terciário do Ceará [Digital]
Dissertação
Português
616.98:578.828Covid19
Fortaleza, 2023.
80f.
A COVID-19, resultante da infecção pelo coronavírus SARS-CoV-2, emergiu como uma significativa preocupação de saúde global no início de 2020, evoluindo rapidamente para uma pandemia. Frente aos progressos já alcançados no conhecimento e controle da COVID-19, é indispensável manter uma evolução...
Ver mais
A COVID-19, resultante da infecção pelo coronavírus SARS-CoV-2, emergiu como uma significativa preocupação de saúde global no início de 2020, evoluindo rapidamente para uma pandemia. Frente aos progressos já alcançados no conhecimento e controle da COVID-19, é indispensável manter uma evolução constante na compreensão científica, visando a uma abordagem holística que otimize estratégias preventivas, diagnósticas e terapêuticas. Objetivou-se avaliar a evolução clínica e expressão de microRNAs de pacientes internados por COVID-19 em hospital público terciário do Ceará. Trata-se de um estudo de coorte com pacientes internados por COVID-19, no período de abril a dezembro de 2020, onde cento e noventa e nove pacientes diagnosticados com COVID-19 foram incluídos no estudo, a partir do qual foram conduzidas duas subavaliações: uma com 43 pacientes que receberam alta hospitalar e outra envolvendo 88 pacientes submetidos a análise genética sanguínea. As avaliações incluíram informações sociodemográficas, clínicas, laboratoriais e análise de micro-RNAs (miR-6501-5p, miR-618 e miR-16-2-3p) relacionadas ao período de internamento hospitalar, assim como foram incluídas as informações relacionadas a persistência de alterações clínicas, incapacidades físicas e psicológicas no trigésimo dia após alta hospitalar. A maioria dos indivíduos eram do sexo masculino, com média de idade de 68,3 anos, portadores de HAS e com uma taxa de mortalidade 60%. Os pacientes que evoluíram para óbito apresentaram uma frequência significativamente aumentada dos sintomas de anorexia e adinamia/fraqueza à admissão, níveis reduzidos de hemoglobina e níveis aumentados de LDH e miR-65015p. Foram também observados níveis significativamente aumentados de miR-6501-5p nos pacientes que foram de alta hospitalar e persistiram com alterações motoras no trigésimo dia pós-alta. Nosso estudo confirma e amplia as características demográficas, clínicas e laboratoriais, disponíveis na literatura, de pacientes hospitalizados por COVID-19. Além disso, constatou-se ineditamente a associação significativa de níveis aumentados de miR-6501-5p com pacientes hospitalizados por COVID-19 que evoluíram à óbito e com a persistência de alterações motoras para aqueles que obtiveram alta hospitalar. É indispensável ressaltar a necessidade de estudos adicionais que aprofundem e validem o papel de microRNAs como biomarcadores no manejo da COVID-19.
Palavras-chave: COVID-19; evolução clínica; biomarcadores; microRNA Ver menos
Palavras-chave: COVID-19; evolução clínica; biomarcadores; microRNA Ver menos
COVID-19, resulting from infection by the coronavirus SARS-CoV-2, emerged as a significant global health concern in early 2020, rapidly evolving into a pandemic. Given the progress already made in the knowledge and control of COVID-19, it is necessary to maintain constant evolution in scientific...
Ver mais
COVID-19, resulting from infection by the coronavirus SARS-CoV-2, emerged as a significant global health concern in early 2020, rapidly evolving into a pandemic. Given the progress already made in the knowledge and control of COVID-19, it is necessary to maintain constant evolution in scientific understanding, adopting a holistic approach that optimizes preventive, diagnostic and therapeutic strategies. The objective was to evaluate the clinical evolution and expression of microRNAs of patients hospitalized
for COVID-19 in a tertiary public hospital in Ceará. This is a cohort study with patients hospitalized for COVID-19, from April to December 2020, where one hundred and ninety-nine published patients with COVID-19 were included in the study, from which
two sub-evaluations were included: one with 43 patients who were discharged from hospital and involved another 88 patients who underwent genetic blood analysis. The assessments included sociodemographic, clinical, laboratory information and analysis
of micro-RNAs (miR-6501-5p, miR-618 and miR-16-2-3p) related to the period of hospital stay, as well as information related to persistence. of clinical changes, physical and psychological disabilities on the thirtieth day after hospital discharge. The majority
of individuals were male, with an average age of 68.3 years, with hypertension and a mortality rate of 60%. Patients who died had a significantly increased frequency of symptoms of anorexia and adynamia/weakness at release, reduced levels of hemoglobin and increased levels of LDH and miR-65015p. Significantly increased levels of miR-6501-5p were also applied to patients who were discharged from hospital and persisted with motor changes on the thirtieth day post-discharge. Our study confirms and expands the demographic, clinical and laboratory characteristics, available in the literature, of patients hospitalized for COVID-19. Furthermore, an unprecedented association was found between increased levels of miR-6501-5p and patients hospitalized for COVID-19 who died and with the persistence of motor changes in those who were discharged from hospital. It is essential to highlight the need for additional studies that deepen and validate the role of micro-RNAs as biomarkers in the management of COVID-19.
Keywords: COVID-19; clinical evolution; biomarkers; micro-RNA. Ver menos
for COVID-19 in a tertiary public hospital in Ceará. This is a cohort study with patients hospitalized for COVID-19, from April to December 2020, where one hundred and ninety-nine published patients with COVID-19 were included in the study, from which
two sub-evaluations were included: one with 43 patients who were discharged from hospital and involved another 88 patients who underwent genetic blood analysis. The assessments included sociodemographic, clinical, laboratory information and analysis
of micro-RNAs (miR-6501-5p, miR-618 and miR-16-2-3p) related to the period of hospital stay, as well as information related to persistence. of clinical changes, physical and psychological disabilities on the thirtieth day after hospital discharge. The majority
of individuals were male, with an average age of 68.3 years, with hypertension and a mortality rate of 60%. Patients who died had a significantly increased frequency of symptoms of anorexia and adynamia/weakness at release, reduced levels of hemoglobin and increased levels of LDH and miR-65015p. Significantly increased levels of miR-6501-5p were also applied to patients who were discharged from hospital and persisted with motor changes on the thirtieth day post-discharge. Our study confirms and expands the demographic, clinical and laboratory characteristics, available in the literature, of patients hospitalized for COVID-19. Furthermore, an unprecedented association was found between increased levels of miR-6501-5p and patients hospitalized for COVID-19 who died and with the persistence of motor changes in those who were discharged from hospital. It is essential to highlight the need for additional studies that deepen and validate the role of micro-RNAs as biomarkers in the management of COVID-19.
Keywords: COVID-19; clinical evolution; biomarkers; micro-RNA. Ver menos
Silva, Raimunda Magalhães da
Orientador
Carioca, Antonio Augusto Ferreira
Coorientador
Bezerra Júnior, Geraldo
Banca examinadora
Garcia Filho, Carlos
Banca examinadora
Silva, Carlos Antônio Bruno da
Banca examinadora
Baixinho, Cristina Rosa Soares Lavareda
Banca examinadora
Barros, Adriana Rolim Campos
Banca examinadora
Vieira, Luiza Jane Eyre de Souza
Banca examinadora