Seeds are one of the main inputs that increase the production costs of potato crops, especially
in tropical and subtropical regions. Aeroponics can be applied for seed production in these
regions, offering a cost-effective alternative. Additionally, plant growth-promoting
microorganisms can enhance plant yield. The objective of this study was to assess whether the
use of plant growth-promoting microorganisms affects the performance of potato cultivars in
aeroponic seed production. The study was divided into three stages: i) in vitro selection of
microorganisms for use in the nutrient solution of aeroponics; ii) assessment of agronomic
variables in potato cultivation with plant growth-promoting microorganisms in aeroponics; iii)
nutritional analysis of minitubers produced in aeroponics inoculated with plant growthpromoting microorganisms. Microorganism survival in the nutrient solution was evaluated in
the laboratory. Three microorganisms with the best results were selected for the second stage:
Bacillus subtilis, Azospirillum brasilense, and Trichoderma sp (D11). By the end of the second
stage, plant roots were found to be colonized by these microorganisms. In general, plant growthpromoting microorganisms increased the number of stolons, initiated minitubers, and harvested
minitubers. However, they did not affect the dormancy period of minitubers. Trichoderma sp.
(D11) increased total yield by 31% compared to the control, reaching 1,176 minitubers per m².
Except for sulfur and manganese, the content of all studied nutrients increased with the use of
microorganisms and may be influenced by the genotype. The use of plant growth-promoting
microorganisms in the aeroponic nutrient solution is promising, as it enhances both the yield
and overall nutritional quality of minitubers. Ver menos